Review



grna scaffold template  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Addgene inc grna scaffold template
    Grna Scaffold Template, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+scaffold+template/pICH86966%3A%3AAtU6p%3A%3AsgRNA_PDS+(Plasmid+%2346966)/pmc12419240-244-31-34
    Average 94 stars, based on 34 article reviews
    grna scaffold template - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Sequencing:

    Article Title: The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism
    Article Snippet: .. For the gRNAs, a 5’ tailed primer harbouring the individual SlFBN spacer (guide sequence), BsaI recognition site and compatible vector overhang was used to generate specific gRNA amplicons by PCR from an gRNA scaffold template (AddGene no. 46966; for primers, see SI Appendix, Table S9). .. Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751, plCH47761, plCH47772, plCH47781, pICH47791, pICH47732, pICH47742, pICH47751) depending on the position of each gRNA in the final binary vector as described by ( ).

    Article Title: The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism
    Article Snippet: .. For the gRNAs, a 5′‐tailed primer harbouring the individual Slfbn spacer (guide sequence), BsaI recognition site and compatible vector overhang was used to generate specific gRNA amplicons by PCR from a gRNA scaffold template (AddGene no. 46966; for primers; see Table ). .. Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751, plCH47761, plCH47772, plCH47781, pICH47791, pICH47732, pICH47742, pICH47751) depending on the position of each gRNA in the final binary vector as described by Lawrenson et al. ( ).

    Plasmid Preparation:

    Article Title: The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism
    Article Snippet: .. For the gRNAs, a 5’ tailed primer harbouring the individual SlFBN spacer (guide sequence), BsaI recognition site and compatible vector overhang was used to generate specific gRNA amplicons by PCR from an gRNA scaffold template (AddGene no. 46966; for primers, see SI Appendix, Table S9). .. Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751, plCH47761, plCH47772, plCH47781, pICH47791, pICH47732, pICH47742, pICH47751) depending on the position of each gRNA in the final binary vector as described by ( ).

    Article Title: The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism
    Article Snippet: .. For the gRNAs, a 5′‐tailed primer harbouring the individual Slfbn spacer (guide sequence), BsaI recognition site and compatible vector overhang was used to generate specific gRNA amplicons by PCR from a gRNA scaffold template (AddGene no. 46966; for primers; see Table ). .. Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751, plCH47761, plCH47772, plCH47781, pICH47791, pICH47732, pICH47742, pICH47751) depending on the position of each gRNA in the final binary vector as described by Lawrenson et al. ( ).

    Polymerase Chain Reaction:

    Article Title: The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism
    Article Snippet: .. For the gRNAs, a 5’ tailed primer harbouring the individual SlFBN spacer (guide sequence), BsaI recognition site and compatible vector overhang was used to generate specific gRNA amplicons by PCR from an gRNA scaffold template (AddGene no. 46966; for primers, see SI Appendix, Table S9). .. Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751, plCH47761, plCH47772, plCH47781, pICH47791, pICH47732, pICH47742, pICH47751) depending on the position of each gRNA in the final binary vector as described by ( ).

    Article Title: The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism
    Article Snippet: .. For the gRNAs, a 5′‐tailed primer harbouring the individual Slfbn spacer (guide sequence), BsaI recognition site and compatible vector overhang was used to generate specific gRNA amplicons by PCR from a gRNA scaffold template (AddGene no. 46966; for primers; see Table ). .. Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751, plCH47761, plCH47772, plCH47781, pICH47791, pICH47732, pICH47742, pICH47751) depending on the position of each gRNA in the final binary vector as described by Lawrenson et al. ( ).



    Similar Products

    sgrna was generated with a pcr-amplified template which contained the t7 promoter and grna scaffold sequences  (Thermo Fisher)
    90
    Thermo Fisher sgrna was generated with a pcr-amplified template which contained the t7 promoter and grna scaffold sequences
    Generation of <t>zebrafish</t> <t>abcc4</t> −/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the <t>sgRNA</t> target sequences for zebrafish abcc4 gene. ( A ) Diagram of the target site in the zebrafish abcc4 genome. ( B ) Abcc4 amino acids of wild-type (WT) and abcc4 −/− mutants. ( C ) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.
    Sgrna Was Generated With A Pcr Amplified Template Which Contained The T7 Promoter And Grna Scaffold Sequences, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+scaffold+template/pmc07923114-173-12-37
    Average 90 stars, based on 1 article reviews
    sgrna was generated with a pcr-amplified template which contained the t7 promoter and grna scaffold sequences - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    grna scaffold template  (Addgene inc)
    94
    Addgene inc grna scaffold template
    Generation of <t>zebrafish</t> <t>abcc4</t> −/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the <t>sgRNA</t> target sequences for zebrafish abcc4 gene. ( A ) Diagram of the target site in the zebrafish abcc4 genome. ( B ) Abcc4 amino acids of wild-type (WT) and abcc4 −/− mutants. ( C ) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.
    Grna Scaffold Template, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+scaffold+template/pICH86966%3A%3AAtU6p%3A%3AsgRNA_PDS+(Plasmid+%2346966)/pmc12419240-244-31-34
    Average 94 stars, based on 1 article reviews
    grna scaffold template - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    grna scaffold-trna template  (GenScript corporation)
    90
    GenScript corporation grna scaffold-trna template
    Generation of <t>zebrafish</t> <t>abcc4</t> −/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the <t>sgRNA</t> target sequences for zebrafish abcc4 gene. ( A ) Diagram of the target site in the zebrafish abcc4 genome. ( B ) Abcc4 amino acids of wild-type (WT) and abcc4 −/− mutants. ( C ) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.
    Grna Scaffold Trna Template, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+scaffold+template/grna+scaffold/pm38564120-85-1-7
    Average 90 stars, based on 1 article reviews
    grna scaffold-trna template - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Generation of zebrafish abcc4 −/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the sgRNA target sequences for zebrafish abcc4 gene. ( A ) Diagram of the target site in the zebrafish abcc4 genome. ( B ) Abcc4 amino acids of wild-type (WT) and abcc4 −/− mutants. ( C ) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.

    Journal: International Journal of Molecular Sciences

    Article Title: Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead

    doi: 10.3390/ijms22042054

    Figure Lengend Snippet: Generation of zebrafish abcc4 −/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the sgRNA target sequences for zebrafish abcc4 gene. ( A ) Diagram of the target site in the zebrafish abcc4 genome. ( B ) Abcc4 amino acids of wild-type (WT) and abcc4 −/− mutants. ( C ) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.

    Article Snippet: The sgRNA target sequences for the abcc4 gene were as follows: GGAGAAGCCGTTCGCCATGC. sgRNA was generated with a PCR-amplified template which contained the T7 promoter and gRNA scaffold sequences, and then synthesized in vitro with T7 RNA polymerase (Thermo Scientific, Waltham, MA, USA).

    Techniques: CRISPR